How to Make Lb Medium

How to Make Lb Mediumの手順についてポイントを解説します。初心者でも安心のガイドをご覧ください。

LB stands for Lysogeny Broth, though your lab mates might call it Luria-Bertani medium. Think of it as the all-purpose stock cube of bacterial cuisine—simple, reliable, and ready to support growth for most non-fussy microbes. Invented by Giuseppe Bertani in 1951, it was originally designed to study lysogeny in bacteriophages. Today, it’s the go-to for cloning, protein expression, and just watching bacteria do their thing.

You’ll see it in two forms: liquid broth for shaking flasks, and solid agar for plates. Both start with the same three core ingredients. Once you know the magic ratio, you’re basically a microbial chef.